In the study, a total of 36 BALB/C mice divided into twelve equal groups were used. Eleven groups were administered 50 μl of inocula containing 107 CFU prepared from eleven isolates of Salmonella Typhimurium, administering one isolate in each group.

One group was kept as negative control and each mouse in this group was given 0.5 ml of normal saline per os.The study was conducted for 15 days during which symptoms viz., ruffl ed feathers, hunched posture, etc., exhibited by the mice were observed and deaths were recorded. At necropsy, gross lesions of moderate to severe focal hepatic necrosis characteristic of Salmonella infection were observed. In some cases, brain and spleen were congested. No signifi cant gross lesions were observed in heart, lung and kidney. Salmonella was isolated from liver, spleen and brain of mice that died1st to 4th day post- exposure. Surviving mice were sacrifi ced on 14th day post- exposure, three of these had necrotic foci on the liver and Salmonella was isolated from all of these mice.